Abstract: In 2000, the State Forestry Administration listed tree shrew as one of the experimental animals in the list of terrestrial wild animals protected by the state, beneficial or of important economic and scientific research value,and it has been widely used in medicine in recent years. It has been confirmed that tree shrews have highly similar brain and visual nerves to humans. At the same time, it is documented that the only animal other than chimpanzees can be infected with human hepatitis B virus. The purpose of this paper is to establish the physiological normal range of artificially raised tree shrews, to screen qualified experimental animals, and to provide basic data for the diagnosis and application of tree shrews. In this experiment, 30 adult western Yunnan subspecies(21 male shrews and 9 female shrews) were artificially raised and bred. The physiological constant index parameters of Western Yunnan subspecies were preliminarily established through the determination of physiological constant indexes;comparative constant parameters of different genders of Western Yunnan subspecies tree shrews; comparison of some physiological constants of blood in western Yunnan subspecies tree shrews with those of rhesus monkeys, rats and ginseng. The results showed that there were significant differences in respiration, erythrocyte sedimentation rate(24 hours), hemoglobin, neutrophilic lobular nucleus leukocytes and leukocyte count between different genders of Western Yunnan subspecies tree shrews(P < 0.05), and there was no significant difference in the other 11 indexes(P < 0.01). Some values of physiological indexes of tree shrews show certain differences with rhesus monkeys, rats and humans(for example, body temperature, respiration and heart rate are higher than those of rhesus monkeys, rats and ginseng).