Abstract: In eco-environmental sensitive areas such as national parks, nature reserves and scenic spots, highways are mostly bypassed by extra-long deep buried tunnels.In the process of tunnel construction, the possible water inrush and leakage will have a serious impact on the water environment, and even lead to the destruction of the ecological environment.In order to study the influence of tunnel construction on surface water resources in ecologically sensitive area, based on the construction of Dulagza tunnel, combined with the engineering geological survey and geophysical exploration and interpretation results of the tunnel site area, through the special investigation of the surface water environment and hydrogeology of the tunnel site area, the analysis method of water diversion fissure zone and 3D geological model were applied to obtain the impact of tunnel construction on surface water sources such as drinking fountain points in the tunnel site, Bigu Tianchi and Qianhu mountain plateau lakes, so as to promote the coordinated development between tunnel construction and environmental protection when providing scientific basis and technical support for tunnel design and construction, ecological environment protection and management.