Abstract: As a new form of tourism, ecotourism in nature reserves is increasingly prosperous, which integrates natural protection, public education and ecological experience. This paper takes Namtso Nature Reserve as the research object, comprehensively analyses the factors existing in the development of ecotourism in Namtso Nature Reserve by SWOT analysis. The results indicate that in the development ecological tourism in Namtso Nature Reserve, the advantages outweigh the disadvantages overall, with more opportunities than challenges. Finally, some suggestion for optimizing the development of ecotourism path are proposed to promote the sustainable development of ecotourism in Namtso Nature Reserve.