Abstract: The nature reserve is the most important, the most essential and the most basic part of the natural ecological space in China.It plays an important role in maintaining national ecological security. Due to some historical reasons, some nature reserve are unclear,the boundary is not clear, the area is not uniform, and some even lack the legal boundary. It is very necessary to carry out delimiting boundary and making markers of the nature reserve. Based on the current situation and existing problems of the demarcation work of the protected area, this paper combs the technical process and key points of the demarcation work of the protected area, as well as the final results of the demarcation, which has certain reference significance for carrying out this work.