Abstract: Along with frequent emerging and wide spreading of wildlife-origin infectious diseases, monitoring and preventing wildlife-borne diseases have become more and more concerned. Various regions have correspondingly increased their investments in monitoring and research on the wild animal diseases. In 2021, Yunnan Forestry and Grassland Bureau initiated a project to establish 10 national standard stations for monitoring diseases of terrestrial animals. Xishuangbanna National Nature Reserve is one of those units to demonstrate development of the national standard monitoring stations in aspects including the site consolidation, storage of emergency supplies, monitoring operations and information sharing. With implementation of the project, a monitoring system with one station, eight monitoring terminates, and one primary laboratory was established; essential filed infra-facilities were consolidated and emergency supplies were stored in place for disease prevention and control. Thirteen monitoring transects of wildlife-origin diseases were optimized and constructed in areas with monitoring priority, and 14 species of mammals and 92 species of birds had been recorded from the transects. Monitoring have been practicing on sources and transmission routes to protect effectively the security of public health from potential and hidden dangers of wild animal epidemics.