Abstract: Natural protected areas are the core carrier of ecological construction,the valuable treasure of Chinese nation,and the important symbol of beautiful China.It play an important role in protecting biodiversity,improving ecological environment quality and maintaining national ecological security.Carrying out the delimiting boundary and making marker of natural protected areas will help promote the standardized construction and refined management of nature reserves,however,due to technological or historical reasons,some natural protected areas’s boundary have blurred,and its rights and liabilities are unclear,and its contradiction between protection and development is prominent.It urgently needs to carry out the delimiting boundary and making marker for natural protected reserves.This paper starts from the current situation of natural protected areas,the task and the existing problems of the delimiting boundary and making marker.It discusses the workflow for this work and gives some suggestion Taking Wenshan National Nature Reserve in Yunnan as the research area,engineering practice was carried out in accordance with laws and regulations.New methods and technologies of surveying and mapping were used to solve the problems and difficulties encountered in this work,and we got a good practical results.It has important promotion and guiding significance for carrying out the delimiting boundary and making marker of other natural protected areas.