Abstract: At present, with the increasing pressure of population growth and rapid economic development, it is extremely urgent to enhance public awareness of conservation and strengthen nature education in protected areas such as national parks. The investigation of national park's natural education resources is the premise and foundation of national park's natural education, so the construction of natural education resources investigation system is undoubtedly of great significance. In order to lay a foundation for the implementation of natural education activities in national parks, this paper establishes a five-in-one investigation system based on site investigation, resource classification, resource distribution, resource inventory and resource evaluation.