Abstract: Based on GIS technology and the land use data of Cangshan section of Cangshan Erhai National Nature Reserve in Yunnan Province in 2000, 2010 and 2019, human disturbance index was used to analyze the dynamic changes of human disturbance in this section. The results showed that:(1) the section of Cangshan reserve was mostly undisturbed or weakly disturbed, accounting for about 95%, and the land affected by other disturbances was about 5%. The section of Cangshan reserve as a whole was mildly disturbed;(2) the human disturbance index of the study section showed an increasing trend with the change of time, and the sections that contributed more to the increase of the index were the buffer section and the experimental section. On the whole, the location of human disturbance section in Cangshan section was basically fixed. With the continuous improvement of land and resources monitoring technology and the strengthening protection, the disturbance degree in this region will be gradually reduced in the future. In the later period of time, some conflicts and problems in the past can be reasonably solved through the integration and optimization of natural protected sections, so as to further reduce the disturbance of human activities to the protected sections.